Figure 2.

4T1 tumor cell line growth in scaffold three-dimensional (3D) and plate 2D conditions exposed to (A) X-ray irradiation (0.5–5 Gy) and (B) 4-hydroxycyclophosphamide (1–100 μg/mL). 4T1 cells were protected against higher doses of X-ray (2.5–5 Gy) cultured on 3D collagen–chitosan scaffolds in comparison with 2D culture conditions (p < 0.05). Tumor cells also were more resistant against 4-hydroxycyclophosphamide when they were cultured on collagen–chitosan scaffolds (p < 0.05). scaff = scaffold, ^*: p < 0.05, ^**: p < 0.01.