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Precultured 4T1 cells were injected into Balb/c mice then mice splenocytes proliferation rates were evaluated on Day 35. A higher splenocytes proliferation rate was observed in 4T1-scaffold precultured group and also preradiated cell groups [both two-dimensional (2D) and 3D cultured cells], in comparison with naïve mice splenocytes. * p < 0.05. radi = radiation; Scaff = scaffold; SD = standard deviation; SI = survival index.
