TABLE 4.
Heme spin-state shifts of WT P450 BM-3 and mutants on the binding of PAHs, and dissociation constants
| Substrate | Heme spin-state shift (%)
|
Kd (μM)
|
||||||
|---|---|---|---|---|---|---|---|---|
| WT | F87V | F87V/L188Q | A74G/F87V/L188Q | WT | F87V | F87V/L188Q | A74G/F87V/L188Q | |
| Naphthalene | 14.5 ± 2.8 | 72.5 ± 2.9 | 67.8 ± 3.0 | 68.4 ± 3.4 | 143 ± 10 | 150 ± 8 | 162 ± 9 | 141 ± 7 |
| Fluorene | 18.8 ± 1.9 | 22.0 ± 3.7 | 14.5 ± 2.7 | 25.9 ± 4.7 | 7.2 ± 1.4 | 6.8 ± 2.1 | 6.4 ± 1.9 | 12.1 ± 2.2 |
| Acenaphthene | 14.1 ± 2.4 | 37.1 ± 2.2 | 37.3 ± 4.1 | 36.5 ± 4.0 | 6.8 ± 1.1 | 24.4 ± 2.9 | 22.1 ± 2.2 | 23.7 ± 3.1 |
| Acenaphthylene | 14.9 ± 2.9 | 44.5 ± 4.1 | 40.8 ± 3.7 | 36.4 ± 3.6 | 24.7 ± 4.9 | 30.5 ± 6.8 | 17.9 ± 1.2 | 30.1 ± 2.5 |