FIG. 6.

Effects of mixed templates on signal intensity. The nirS gene from P. stutzeri E4-2 was labeled with Cy3 using PCR amplification and nirS-specific primers. The signal intensity data presented in the filled bars were from hybridization experiments with the Cy5-labeled E4-2 nirS gene, while the data in open bars were from hybridization experiments with Cy5-labeled E4-2 nirS gene plus an equal amount of the D8-12 nirS gene. The nirS genes of E4-2 and D8-12 are 92% identical.