FIG. 5.

Intestinal FXR/FGF15 and inflammation are regulated by MC-FXR. Introduction of MCs in Kit^W-sh mice increases ileal (A) FXR and (B) FGF15 expression compared with WT and Kit^W-sh mice and inhibition of MC-FXR decreases (A) FXR and (B) FGF15 expression, as shown in Kit^W-sh + MC-Gugg mice. (C) MC injection increased small intestine expression of SCF, IL-1β, TGF-β1, and CCL3 in Kit^W-sh compared with WT and Kit^W-sh mice; however, intestinal inflammation was reduced when MC-FXR was inhibited. Representative images are presented as ×20. Data are mean ± SEM of n = 4 experiments for qPCR from 6–8 mice (MC injection group). *P < 0.05 vs. WT and Kit^W-sh, #P < 0.05 vs. Kit^W-sh + MC, &P < 0.05 vs. WT.