Table 3.
Identification of histamine-forming bacteria isolated from milkfish dumpling samples by 16S rDNA, based on the output results from NCBI database analysis, and their production of histamine and other biogenic amines in culture broth.
| Strain | Organism identified | Percentage identity (%) | GenBank accession number | Biogenic amine production (ppm) | |||
|---|---|---|---|---|---|---|---|
|
| |||||||
| His | Cad | Spd | Spm | ||||
| MFS5 | Raoultella ornithinolytica | 99 | AB682291.1 | 276.6 | 194.0 | NDa | ND |
| MFS8 | Raoultella ornithinolytica | 99 | JF701186.1 | 561.8 | 205.5 | 0.5 | 0.1 |
| MFS9 | Enterobacter aerogenes | 99 | CP002824.1 | 404.0 | 42.0 | 27.0 | 26.0 |
a
ND, not detected (amine level < 0.05 ppm).
Cad = cadaverine; His = histamine; Spd = spermindine; Spm = spermine.