Figure 5.

Full-length Secisbp2l but not CT-Secisbp2l interact with eEFSec. A, eEFSec was not co-immunoprecipitated with CT-Secisbp2l. Expression of full-length Secisbp2l (Flag-Secisbp2l) was undetectable. B, Strategy of detecting protein-protein interactions by split mCherry. When the fusion proteins interact with each other, the split mCherry restores its fluorescence. C, Scheme of fusion constructs used for split mCherry experiments. Note that the protein size was not scaled proportionally. D, CN-eEFSec was co-transfected with constructs encoding various Secisbp2l fragments. Only the full-length but not the CT-Secisbp2l interacts with eEFSec and restored mCherry activity. GFP expression regulated by an independent promoter was used as an internal control. Exposure time for capturing GFP and mCherry images was 40 ms and 1 s, respectively.