Figure 6.

Disruption of Secisbp2l reduced T4-mediated differentiation of CG4 cells. A, Cas9-mediated Secisbp2l mutation. 10 out of the 20 amplified DNA fragments contained the Secisbp2l mutation, indicating that ∼50% of Secisbp2l in CG4 cells was mutated. The targeting sequence is underlined, and the PAM sequence is bolded. Note that the letter G in blue is variant from T in GenBank. B, C, Disruption of Secisbp2l reduced the differentiation of CG4 cells in response to T4 but not T3 treatment. D, Altered T4 consumption activity in Secisbp2l-expressing or Secisbp2l-deficient CG4 cells. Data were showed as means ± SD; t test was used for statistical analysis based on triplicate results.