Figure 7.

Secisbp2l CKO results in decreased selenoprotein levels. A, Schematic representation of Secisbp2l-CKO generation. B, Western blot confirmed that full-length SECISBP2L (indicated by an arrow) was absent in P3 Secisbp2l-CKO spinal cord, coupled with a dramatically reduced level of DIO2 and SELENOP. C, D, IF verified the loss of Secisbp2l+ cells in Secisbp2l-CKO mice from P3 to P15. E–G, DIO2 was drastically reduced in OLIG2+ OLs in Secisbp2l-CKO as revealed by double immunostaining. All values are represented as mean ± SD; ***p < 0.001; NS, not significant.