Figure 9.

Secisbp2l-CKO causes reduced OL differentiation and myelin deficit. A–D, ISH revealed a normal number of Pdgfra+ OPC in Secisbp2l-CKO mice from P0 to P15 (A, B), but the number of mature Plp1+OLs is significantly reduced at all tested stages (C, D). Values are represented as mean ± SD (B, D). E, F, Transmission electron microscopy of P15 spinal cord showed thinner myelin sheaths in Secisbp2l-CKO mice (E) and increased G-ratios (F), G-ratios were calculated from pooled data from three animals for both control and Secisbp2l-CKO mice. G, Decreased differentiation of OL in brain tissues from Secisbp2l CKO mice. ISH revealed a reduced expression of the mature OL markers Mbp and Plp1 in Secisbp2l-CKO mice.