Fig. 1. TEM image and DLS data of (a) WP5⊃G and (b) WP5⊃G–siRNA nanovesicles. (c) Tyndall effects of WP5, WP5⊃G and WP5⊃G–siRNA. (d and e) HAADFS-TEM images and area elemental mappings of G–siRNA and WP5⊃G–siRNA. (Representative elements: N and O for WP5; G for siRNA; P for siRNA). (f) The zeta potential of G, WP5⊃G and WP5⊃G–siRNA in water (n = 3). (g(i)) Absorption spectra of the complex WP5⊃G with different molar ratios in water ([WP5] + [G] = 20 μM, 298 K, and wavelength range 220–650 nm); (ii) Job plot of WP5⊃G showing a 2 : 1 stoichiometry. (h) The critical aggregation concentration of the WP5⊃G complex.
