Distribution of the different S states (% of the total PSII amount) in EPR samples after the application of 0–6 turnover flashes given at frequency of 5 Hz and 10 Hz at 1 °C (see ESI Fig. 1)a.
Fl. freq. | Fl. no. | S1 | S2 | S3 | S0 | S1(2nd) | S2(2nd) | S3(2nd) | Total |
---|---|---|---|---|---|---|---|---|---|
5 Hz | 0 | 100 | 100 | ||||||
1 | 100 | 100 | |||||||
2 | 35 ± 1 | 65 ± 1 | 100 | ||||||
3 | 16 ± 2 | 29 ± 2 | 57 ± 1 | 102 | |||||
4 | 11 ± 2 | 13 ± 1 | 26 ± 1 | 49 ± 1 | 99 | ||||
5 | 6 ± 2 | 15 ± 1 | 28 ± 1 | 53 ± 3 | 102 | ||||
6 | 6 ± 1 | 10 ± 1 | 44 ± 2 | 40 ± 1 | 100 | ||||
10 Hz | 0 | 100 | 100 | ||||||
1 | 100 | 100 | |||||||
2 | 43 ± 2 | 57 ± 1 | 100 | ||||||
3 | 24 ± 2 | 28 ± 1 | 48 ± 1 | 100 | |||||
4 | 17 ± 1 | 13 ± 2 | 33 ± 3 | 37 ± 3 | 100 | ||||
5 | 6 ± 1 | 22 ± 1 | 23 ± 3 | 46 ± 1 | 97 | ||||
6 | 14 ± 1 | 14 ± 1 | 41 ± 1 | 29 ± 3 | 98 |
The fraction of the Si – state was determined from the EPR spectra as described in the text and in ref. 40.