FIGURE 2.

Factors controlling the position of bleb formation. (A) Global hydrostatic pressure induces bleb formation at locations where the actin cortex is disrupted. (B) Preferential initiation of blebs at locations with reduced levels of membrane linker molecules. (C) Locally increased contractility could induce bleb formation at these loci if poroelastic properties of the cytosol prevent rapid pressure equilibration. (D) Depending on membrane curvature, the force resulting from membrane tension is either directed inward in the case of positive membrane curvature (left) or outward for negative membrane curvature (right). Therefore, membrane delamination occurs more readily at locations of negative membrane curvature.