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. 2022 Jul 29;8(30):eabn7702. doi: 10.1126/sciadv.abn7702

Fig. 1. More SAC-positive kinetochores in modern human than chimpanzee APs.

Fig. 1.

(A to C) APs in metaphase stained with DAPI (4′,6-diamidino-2-phenylindole) and immunostained for the SAC marker BubR1 (red in merges) and the kinetochore marker CREST (green in merges). Arrowheads indicate overlap of BubR1 and CREST immunoreactivity within the metaphase plate. (A) GW12 human neocortex; (B) day 30 human iPSC-derived cerebral organoid; and (C) day 30 chimpanzee iPSC-derived cerebral organoid. White dashed lines, ventricular surface. Scale bar, 5 μm. (D) Quantification of kinetochores positive for BubR1 per AP metaphase plate, for the tissues in (A) to (C) (GW11 to GW12, days 30 to 32). Data are the means ± SEM of ≥41 APs from ≥3 independent experiments each, with 3 neocortex samples and ≥5 organoids each. Brackets with **P < 0.01; ***P < 0.001; n. s., nonsignificant (Kruskal-Wallis test with Dunn’s multiple comparisons correction).