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. 2022 Jul 29;8(30):eabn7702. doi: 10.1126/sciadv.abn7702

Fig. 6. Fewer lagging chromosomes in humanized mouse APs, and more lagging chromosomes in ancestralized human organoid APs.

Fig. 6.

(A to H) Mitotic APs stained with DAPI in the neocortex of E11.5 wt mice and mice carrying humanized hKif18a-hKnl1 (A to D) and in days 27 to 30 control cerebral organoids (line 2) and organoids with ancestralized aKIF18a-aKNL1 (line 2, E to H). Examples of APs in anaphase (A and E) and telophase (B and F) without lagging chromosomes, and in anaphase (C and G) and telophase (D and H) with lagging chromosomes, or large chromosome fragments (arrowheads). White dashed lines, ventricular surface. Scale bar, 5 μm. (I) Cumulative quantification (see Materials and Methods) of the percentages of AP divisions with lagging chromosomes for the tissues described in (A) to (H), as well as for the neocortex of E11.5 mice carrying humanized hKif18a-hKnl1-hSpag5. The mouse data are the sum of ≥314 AP divisions in anaphase or telophase from six experiments for each of the three types of mouse neocortex, and the human organoid data are the sum of ≥389 AP divisions from six experiments for each of the two types of organoids (sums of lines 1 and 2). The percentages for telophase (dark shade) and anaphase (light shade) are indicated separately.