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. 2022 Jul 29;8(30):eabn5360. doi: 10.1126/sciadv.abn5360

Fig. 6. In vivo implantation of cast gel disk scaffolds.

Fig. 6.

(A) Gel scaffolds were subcutaneously implanted on the dorsa of Sprague-Dawley rats and harvested at various time points. (B) Macro view and hematoxylin and eosin (HE) staining images of gels at days 7 and 14 after implantation. Scale bars, 5 mm (macro view) and 50 μm (HE staining). The dashed line in HE staining: The deepest cell penetration position in scaffolds. (C) Processed images of HE staining showed cellular penetration of nuclei (white point) through gel scaffolds. Scale bars, 100 μm. (D) Mean cell density as a function of penetration depth into the gel scaffolds at 7 days after implantation.