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. Author manuscript; available in PMC: 2022 Jul 29.
Published in final edited form as: Nat Methods. 2022 Mar 28;19(4):479–485. doi: 10.1038/s41592-022-01427-0

Extended Data Fig. 4. Comparison of HYBRiD and other methods on clearing of newborn mice.

Extended Data Fig. 4

A,B. Representative brightfield images of PV-Ai9 newborn mice processed by indicated clearing methods and RI matched in the indicated media (3 samples per condition). Grid size: 3 mm. C - E. Overview of lightsheet imaging of PV-Ai9 newborn mice cleared by the indicated clearing protocols. 3D volume of cleared PV-Ai9 mice from each clearing protocol is shown in coronal view (C), sagittal view (D), and transverse view (E). Note HYBRiD, SHANEL, and uDISCO sample were imaged fully through the D-V axis. D, dorsal; V, ventral; S, superior; I, inferior; L, lateral; M, medial. Scale bar: 2 mm.