Sample Type | Fixation | Pre-Treatment | Organic Clearing | Embedding | Aqueous Clearing* | Immunostaining | Refractive Index Match |
---|---|---|---|---|---|---|---|
1mm Brain Sections | 1 d | - | 1 d | 2 d | 7 d | - | 1 d |
Whole Mount Limbs | 1 d | 6 d | 1 d | 2 d | 14 d | - | 2 d |
Newborn Whole Body | 2 d | 6 d | 2 d | 3 d | 21 d | - | 2 d |
Adult Chest (without perfusion) | 4 d | 10 d (4+6) | 2 d | 3 d | 28 d | 8 d | 3 d |
Juvenile Whole Body (perfused) | 2 d | 8 d (4+4) | 3 d | 3 d | 28 d | - | 3 d |
Optional use of active clearing will shorten this step by 25–50%