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. Author manuscript; available in PMC: 2022 Jul 29.
Published in final edited form as: Nat Methods. 2022 Mar 28;19(4):479–485. doi: 10.1038/s41592-022-01427-0
Sample Type Fixation Pre-Treatment Organic Clearing Embedding Aqueous Clearing* Immunostaining Refractive Index Match
1mm Brain Sections 1 d - 1 d 2 d 7 d - 1 d
Whole Mount Limbs 1 d 6 d 1 d 2 d 14 d - 2 d
Newborn Whole Body 2 d 6 d 2 d 3 d 21 d - 2 d
Adult Chest (without perfusion) 4 d 10 d (4+6) 2 d 3 d 28 d 8 d 3 d
Juvenile Whole Body (perfused) 2 d 8 d (4+4) 3 d 3 d 28 d - 3 d
*

Optional use of active clearing will shorten this step by 25–50%