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. 2022 Jul 4;145(7):2313–2331. doi: 10.1093/brain/awac082

Figure 2.

Figure 2

Loss of PIK3-C2β activity causes mTORC1 hyperactivation in vitro. (A) Representative immunoblot of PI3K-C2β variants transfected in HEK293T cells immunoprecipitated with GFP antibody (IP), as used for the kinase assay, and total lysates (TL). (B) Quantification of the kinase activity on PI(4)P of PI3K-C2β URVs, gnomAD variants (grey bars) and controls compared to WT PI3K-C2β. One-way ANOVA, *P < 0.05, **P < 0.01. n > 3 independent experiments. Error bars represent SEM. (C) Representative immunoblot of GFP, PI3K-C2β-GFP, p-S6K, S6K and GAPDH content in HEK293T cells transfected with GFP, WT PI3K-C2β, PI3K-C2β URVs (left), or gnomAD variants (right). (D) Quantification of the p-S6K/total S6K of data shown in C. One-way ANOVA, *P < 0.05, **P < 0.01. n > 3 independent experiments. Error bars represent SEM.