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. 2022 Jul 4;145(7):2313–2331. doi: 10.1093/brain/awac082

Figure 5.

Figure 5

Dose-dependent PI3K-C2β expression predisposes to pilocarpine-induced epileptic seizure s. (A) Severity of seizure behaviour over time following intraperitoneal injection of 350 mg/kg pilocarpine in 2–3-month-old male Pik3c2b KO, HET and littermate WT mice. Higher scores correspond to more severe seizure status; a score of 6 indicates status epilepticus, a score of 7 indicates mortality. Two-way ANOVA, **P < 0.01. n = 9 WT, n = 10 HET, n = 9 KO. Error bars represent SEM. (B) Incidence of seizures in KO, HET and WT mice. n is as in A. (C) Severity of seizure behaviour over time following intraperitoneal injection of 350 mg/kg pilocarpine in 2–3-month-old Pik3c2b KO, HET and littermate WT mice pretreated with 10 mg/kg everolimus (Ev) 90 min before pilocarpine injection. Higher scores correspond to more severe seizure status; a score of 6 indicates status epilepticus, a score of 7 indicates mortality. Two-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001. n = 9 WT, n = 10 HET, n = 9 KO. Error bars represent SEM. (D) Incidence of seizures in KO, HET and WT mice treated with everolimus. n is as in C. (E) Time needed for experimental groups to reach seizure stage 4 (forelimb clonus, rearing and falling). One-way ANOVA, *P < 0.05 n > 9 for each experimental group. Error bars represent SEM. (F) Maximal severity stage reached by experimental groups at the end of the behavioural observation. n as in A and C. (G) Kaplan–Meier survival curve for untreated (continuous line) or everolimus-treated (dotted line) WT, HET and KO littermates injected with 350 mg/kg pilocarpine. P < 0.0001. n as in A and C.