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. 2022 Aug 1;132(15):e157678. doi: 10.1172/JCI157678

Figure 1. Early infiltration of CD8+ TRLs provides protection against cerebral ischemia.

Figure 1

(A) CD8+CD122+ T cell infiltration in the ipsilateral and contralateral brains at indicated time points after 60-minute tMCAO. n = 4–7/group. Two-tailed Student’s t test. (B) Expression of CD103, IL-10, and HELIOS in brain-infiltrating CD8+CD122+ T cells and CD8+CD122– T cells 3 days after tMCAO. n = 4/group. Two-tailed Student’s t test. (C) Most of the infiltrating CD8+CD122+ T cells are CD49dlo TRLs. (D–H) Mice were treated with isotype IgG (100 μg) or anti-CD122 mAb (100 μg) 2 days prior to 60-minute tMCAO. n = 8–12/group. (D) Anti-CD122 mAb treatment depleted CD8+CD122+ T cells in blood 1 day after stroke. (E) Quantitative measurements of cortical CBF before MCAO (baseline), during MCAO, and 15 minutes after reperfusion. (F) Representative images of T2 MRI scans 1, 3, and 14 days after tMCAO. The yellow dotted lines depict the infarct areas. Quantification of brain infarcts (G) and brain edema (H). In G, black symbols indicate IgG treatment, red symbols indicate anti-CD122 treatment, and P = 0.06 for anti-CD122 versus IgG at 3 days after tMCAO. Two-way (E and H) or mixed-effects (G) repeated measures ANOVA and post hoc Bonferroni’s test. (I) FACS-isolated CD8+ TRLs were labeled with CFSE and adoptively transferred (1 million cells, i.v.) to recipient mice 48 hours after anti-CD122 mAb injection. CD8+CD122+CFSE+ cells were detected in blood 24 hours after cell transfer. (J) Adoptive transfer of CD8+ TRLs 2 hours after tMCAO reversed the detrimental effects of anti-CD122 mAb on brain infarction 3 days after tMCAO as shown by MAP2 staining. n = 6/group. One-way ANOVA and post hoc Dunnett’s test. Scale bar: 1 mm. (K) Quantification of infarct volumes 3 and 7 days after tMCAO in mice treated with IgG, anti-CD122 mAb, or anti-CD25 mAb (100 μg). n = 7–8/group. One-way ANOVA and post hoc Dunnett’s test. (L–N) Sensorimotor function was analyzed with the rotarod test (L), adhesive removal test (M), and foot-fault test (N). n = 8–10/group. Two-way repeated measures ANOVA and post hoc Dunnett’s test. *P < 0.05, **P < 0.01, ***P < 0.001 for anti-CD122 vs. IgG. #P < 0.05, ##P < 0.01, ###P < 0.001 for anti-CD25 vs. IgG.