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Details of essential genes.
| Gene symbol | Description | Rank in the feature list |
| Lhx8 | LIM homeobox 8 | 2 |
| Calm1 | Calmodulin 1 | 4 |
| Hmgb1 | High mobility group box 1 | 9 |
| Meis2 | Meis homeobox 2 | 12 |
| Nr2f2 | Nuclear receptor subfamily 2 group F member 2 | 22 |
| Basp1 | Brain abundant membrane attached signal protein 1 | 7 |
| Actb | Actin beta | 23 |
| Zic1 | Zic family member 1 | 30 |
| Nr2f1 | Nuclear receptor subfamily 2 group F member 1 | 15 |
| Rps29 | Ribosomal protein S29 | 18 |
