Figure 2.
Stromal marker expression in stromal Tcf21-deficient kidneys at E14.5 and E16.5. (A and A′) Slug expression is diminished in Foxd1Cre;Tcf21f/f kidneys at E14.5 compared with the wild type (WT; immunostaining). (B and B′) In contrast, the expression of Foxd1 (evaluated by staining for GFP in Foxd1-eGFPCre;Tcf21f/f and Foxd1-eGFPCre;Tcf21f/+) is not changed in the mutant. (C and C′) At E16.5, Slug expression is dramatically reduced in the medullary stroma in the stromal Tcf21 knockout kidneys. (D and D′) The Acta2 is severely reduced in the interstitial cells in the medulla of the stromal Tcf21 knockout kidney. (E) quantification of Slug+ area normalized to total kidney area in Foxd1Cre;Tcf21f/f kidneys and WT control. Scale bar 100 μm; P=0.03.