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Stromal Tcf21-deficient kidneys have lower β-catenin protein expression in the medullary stroma. (A, A′, B, and B′) β-Catenin protein expression is markedly reduced in the interstitium of the Foxd1Cre;Tcf21f/f kidney at E16.5 and E14.5 (immunostaining). (C and C′) Immunoprecipitation of Tcf21 of in MK3 cells showed direct interaction between Tcf21 and β-catenin, in both the presence and absence of β-catenin stabilization with LiCl2. Scale bar 100 μm.
