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. Author manuscript; available in PMC: 2023 Mar 17.
Published in final edited form as: Cell. 2022 Feb 24;185(6):1082–1100.e24. doi: 10.1016/j.cell.2022.01.023

Figure 4. Spatial organization of mitochondria and synapses in L2/3 PyCs.

Figure 4.

(A) Mitochondrial segmentation cutout.

(B) PyC 3D rendering with mitochondria. Insets show the soma (top) and axon (bottom).

(C) Comparison of mitochondrial volume by compartment (Naxonal=11,484, Nsomatic=90,193, Napical=18,608, Nbasal=53,318 from 351 PyCs; One-tailed Mann-Whitney U tests; *:p<1×10−86, **:p≈0). Boxes show the IQR, and whiskers show the 5th and 95th percentiles.

(D-E) Mitochondrial volume per unit length (D) and mitochondrial path length per unit length (E) at different distances to soma.

(F) Median overlap (red) or uncovered (blue) path length within dendritic segments.

(G-I) Median dendrite diameter (G), linear synapse density (H), and areal synapse density (I) at different distances to soma.

(J) Coefficient of variation for linear (red) and areal (blue) synapse density at different distances to soma. Dots and shaded regions in D, E, G, H, and I show mean +/− SD at each distance bin. N=29,591.Scale bars: (A) 1.5 μm (B) 10 μm, top inset 5 μm, bottom inset 2.5 μm.

See also Figure S2.