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. 2022 Jul 29;12:13082. doi: 10.1038/s41598-022-17023-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Roundup Ultra Plus (RUP), glyphosate (GLY) and surfactant polyoxyethylene tallow amine (POEA) on acrosome membrane damage (A) and phosphatidyl-serine exposure in the plasma membrane (B) in human spermatozoa. Spermatozoa were incubated in BWW for 1 h at 37 °C, 5% CO₂, in the absence or presence of indicated concentrations of RUP, POEA, GLY or POEA in combination with GLY. The results were expressed as the mean of the percentage of PNA-FITC-positive and PI-negative spermatozoa (n = 5) (A), and of Annexin V- FITC positive and PI-negative spermatozoa (n = 4) (B) ± standard error of the mean (SEM). No statistical differences were found.