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. 2022 Jul 29;12:13082. doi: 10.1038/s41598-022-17023-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Roundup Ultra Plus (RUP), glyphosate (GLY) and surfactant polyoxyethylene amine (POEA) in the percentages of motile (A) and type a + b human spermatozoa (B). Human spermatozoa were incubated 1 h in BWW at 37 °C, 5% CO₂, in the absence or the presence of indicated concentrations of RUP, GLY and POEA. Each experiment was performed 13 times (n = 13) and results were normalized and expressed as percentage of control in both cases ± standard error of the mean (SEM). Columns with different letters are statistically different from each other, so that for P < 0.05.