Table 1.

List of primers designed for accurate species identification in the multiplex PCR assay.

Primer pair	Sequence (5’–3’)	Amplicon size (bp)
Opsin4_capitata_F	GCTAAAGCCATAACAATTCAG	327
Opsin4_capitata_R	CAGACTGTTCTTTTGGGC
Opsin4_cosyra_F	GCTGTGACTTTGTTACAG	183
Opsin4_cosyra_R	GCATACTTGAATCTCAATCGAA
Opsin4_quilicii_F	GCGTTCTGTTTTTAATCACTCA	128
Opsin4_quilicii_R	CATTTAATGTTTCAGAAGTGCT
Opsin4_rosa_F	ATTGCTACAACTTTGTCGC	249
Opsin4_rosa_R	GCAGTAATACTGCGAATCATC
Opsin4_dorsalis_F	TAGCACAATTATTTAGCGGG	676
Opsin4_dorsalis_R	ATTACCGTCAGCGATCAG