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. 2022 Jul 29;13:4395. doi: 10.1038/s41467-022-32127-0

Fig. 1. GBP1, GBP2, GBP3, and GBP5 are required for F. novicida-induced inflammasome activation.

Fig. 1

a Immunoblot analysis of caspase-1 (Casp-1) and gasdermin D (GSDMD) in WT, Gbp1–/–, Gbp2–/–, Gbp3–/–, Gbp5–/–, Gbp7–/–, Gbpchr3-KO, or Aim2–/– BMDMs left untreated (Med.) or assessed after infection with F. novicida (MOI 100) for 10 h. b The release of IL-1β and IL-18 from BMDMs after treatment as in a. c The release of LDH from BMDMs after treatment as in a. d Immunoblot analysis of Casp-1 and GSDMD in WT, Gbp1–/–, Gbp3–/– and Aim2–/– BMDMs left untreated (Med.) or assessed after infection with F. novicida (MOI 100) for 10 h, infection with L. monocytogenes (MOI 100) for 20 h, infection with MCMV (MOI 10) for 10 h or transfection of poly(dA:dT) (5 µg/ml) and pcDNA (5 µg/mL) for 4 h. e The release of IL-1β, IL-18 and LDH from BMDMs after treatment as in d. f Light microscopy analysis of WT, Gbp1–/–, Gbp3–/– or Aim2–/– BMDMs left untreated (Med.) or following infection with F. novicida as in d. White arrows indicate pyroptotic cells. Each symbol represents an independent experiment (b, c, and e). Scale bar, 20 µm (f). ns no statistical significance; **P < 0.01; ***P < 0.001; ****P < 0.0001 (one-way ANOVA with Dunnett’s multiple-comparisons test (b, c, and e)). Data are representative of three independent experiments (af; mean and s.e.m. in b, c, and e). Source data are provided as a Source data file.