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. 2022 Jul 29;13:4395. doi: 10.1038/s41467-022-32127-0

Fig. 5. GBP1, GBP2, GBP3 and GBP5 provide host protection against F. novicida infection in vivo.

Fig. 5

a Survival of 7-week-old WT mice (n = 25), Gbp1–/– mice (n = 25) and Aim2–/– mice (n = 32) infected subcutaneously with 1.2 × 106 colony-forming units (CFUs) of F. novicida. b Body weight of 7-week-old WT mice (n = 25), Gbp1–/– mice (n = 18) and Aim2–/– mice (n = 25) 0–7 d after subcutaneous infection with 1.2 × 106 CFUs of F. novicida, presented relative to initial body weight at day 0, set as 100%. c Survival of 7-week-old WT mice (n = 15), Gbp3–/– mice (n = 15) and Aim2–/– mice (n = 10) infected subcutaneously with 1.2 × 106 colony-forming units (CFUs) of F. novicida. d Body weight of 7-week-old WT mice (n = 15), Gbp3–/– mice (n = 15) and Aim2–/– mice (n = 7) 0–7 d after subcutaneous infection with 1.2 × 106 CFUs of F. novicida, presented relative to initial body weight at day 0, set as 100%. e Bacterial burden in the liver (left) and spleen (middle) and concentration of IL-18 in the serum (right) of 7-week-old WT mice (n = 17), Gbp1–/– mice (n = 15) and Aim2–/– mice (n = 16) on day 3 after infection with 6 × 105 CFUs of F. novicida. f Bacterial burden in the liver (left) and spleen (middle) and concentration of IL-18 in the serum (right) of 7-week-old WT mice (n = 14), Gbp3–/– mice (n = 9) and Aim2–/– mice (n = 9) on day 3 after infection with 6 × 105 CFUs of F. novicida. g Bacterial burden in the liver (left) and spleen (middle) and concentration of IL-18 in the serum (right) of 7-week-old WT mice (n = 10) and Gbp2–/– mice (n = 9) on day 3 after infection with 6 × 105 CFUs of F. novicida. h Bacterial burden in the liver (left) and spleen (middle) and concentration of IL-18 in the serum (right) of 7-week-old WT mice (n = 10) and Gbp5–/– mice (n = 12) on day 3 after infection with 6 × 105 CFUs of F. novicida. Each symbol represents an individual mouse (e–h). ns no statistical significance; *P < 0.05, **P < 0.01; ***P < 0.001, ****P < 0.0001 (log-rank test (a and c) or one-way ANOVA with Dunnett’s multiple-comparisons test (e, f, mean and s.e.m. in e, f)) or two-tailed t-test (b, d, g, h, mean and s.e.m. in b, d, g, h). Data are pooled from two or three independent experiments (a–h). Source data are provided as a Source data file.