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. 2022 Jul 29;8:341. doi: 10.1038/s41420-022-01133-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A, B S-24, ZH-161, ZH-305, or T-325 human GIC were assessed for expression of CD95 or CD95L mRNA by RT-qPCR using primers targeting all known protein-coding CD95 or CD95L transcript variants and ARF1 as an internal control. Phorbol myristate acetate (10 ng/ml) and ionomycin (500 ng/ml)-activated peripheral blood mononuclear cells (PBMC) served as a positive control for CD95L expression detection. RT-qPCR data are expressed as mean and SD. a.t., above the threshold, indicates C_T values above the reliability threshold of 32. C, D Cell surface protein levels were assessed by flow cytometry. Specific fluorescence indexes (SFI) were calculated by dividing the median fluorescence intensities of the experimental antibody and the isotype control.