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. 2022 Jul 29;12:13069. doi: 10.1038/s41598-022-17339-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Five samples for the P.1 + P681H, B.1.351, B.1.1.7, B.1.258 and WT were analyzed. The melting curve of the five samples are illustrated for the N439K assay. As it is shown in the graph the N439K mutation can be distinguished by approximately 5 °C from the WT sequence.