Fig. 5.

Changes in cardiac metabolite abundances following 1 session of exercise in female murine hearts. Female mice were subjected to 1 bout of high-intensity exercise and hearts were freeze-clamped immediately or 1 h after the exercise bout for unbiased metabolomic analyses. (A) Heatmap of the top 50 most changed metabolites in hearts from exercised versus sedentary female mice; bolded metabolites indicate FDR < 0.10 following one-way ANOVA; (B) Graphs of individual metabolites that showed the most prominent changes caused by exercise; (C) PLS-DA plot; (D) corresponding VIP plot; and (E) pathway impact analysis derived from most significantly changed metabolites. n = 4 female mice per group. 3-OHB = 3-hydroxybutyrate; ANOVA = analysis of variance; Exe = exercise; FDR = false discovery rate; GPC = glycerophosphocholine; GPI = glycosylphosphatidylinositol; PLS-DA = partial least-squares discriminant analysis; VIP = variable importance plot.