KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-Caspase-1 | Santa Cruz | Cat#SC56036 |
| Anti-GAPDH | CST | Cat#2118 |
| Anti-GSMD | Abcam | Cat#ab209845 |
| Anti-APOL1 | Proteintech | Cat#66124–1-Ig |
| Anti-APOL1 | Genentech | Rabmab 5.17D12 |
| Anti-GFP | Abcam | Cat#AB6673 |
| Anti-LC3II | CST | Cat#2775 |
| Anti-CD31 | Abcam | Cat#ab24590 |
| Anti-Icam1 | Abcam | Cat#ab179707 |
| Anti-Vcam1 | Abcam | Cat#ab134047 |
| Anti-Caveolin-1 | CST | Cat#3267 |
| Anti-Enos | Abcam | Cat#ab5589 |
| Anti-Hsp60 | CST | Cat#12165 |
| Anti-Cox4 | R&D Systems | Cat#MAB6980 |
| Anti-STING | CST | Cat#13647s |
| Anti-phosphorylated STING | CST | Cat#19781s |
| Anti-cGAS | CST | Cat#31659S |
| Anti-TBK1 | CST | Cat#3504S |
| Anti-phosphorylated TBK1 | CST | Cat#5483 |
| Anti-IRF3 | CST | Cat#11904T |
| Anti-phosphorylated IFR3 | CST | Cat#37829 |
| Anti-P65 | CST | Cat#5483 |
| Anti-Phospho-NF-κB p65 | CST | Cat#8242 |
| Monoclonal Anti-β-Actin−Peroxidase antibody | Sigma Aldrich | Cat#A3854 |
| Anti-VE-cadherin | Santa Cruz | Cat#sc-6458 |
| OXPHOS antibody cocktail | Abcam | Cat#ab110413 |
| Anti-p62 | CST | Cat#5114 |
| Donkey anti-Rabbit IgG (H+L) Alexa Fluor 555 | Invitrogen | Cat#A31572 |
| Chicken anti-Mouse IgG (H+L), Alexa Fluor 488 | Invitrogen | Cat#21200 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| LPS (Escherichia. coli O26:B6) | Sigma-Aldrich | Cat#L8274 |
| VX765 | Cayman | Cat#CAYM-28825–50 |
| Disulfiram | Cayman | Cat#97–77-8 |
| C176 | Bio Vision | Cat#B2341 |
| Evans blue dye | Sigma | Cat#E2129 |
| CD31 MicroBeads, mouse | miltenyi biotec | Cat#130–097-418 |
| TET system approved FBS | clontech | Cat#631106 |
| Microvascular EBM-2 | Lonza | Cat#cc-3162 |
| Hydrogen peroxide solution 30% | Sigma | H1009–500ML |
| Trizol | Invitrogen | Cat#15596018 |
| cDNA Reverse Transcription Kit | Applied Biosystems | Cat#4368813 |
| Power SYBR® Green PCR Master Mix | Applied Biosystems | Cat#4367659 |
| RIPA buffer | Cell signaling | Cat#9806 |
| Protease cocktail | Roche | Cat#11836153001 |
| BCA protein assay | Thermo Scientific | Cat#23225 |
| Western Blotting Substrate | Pierce | Cat#32106 |
| Lipofectamine 3000 reagents | Thermo Fisher Scientific | Cat#11668019 |
| LysoTracker™ Blue DND-22 | Thermo Fisher Scientific | Cat#L7525 |
| ECIS Cultureware Disposable Electrode Arrays | Applied BioPhysics | 8W10E+ (8 Well PET) |
| l-cysteine | Sigma | Cat#C7352–25G |
| Gelatin solution | Sigma | Cat#G1393–100ML |
| JC-1 | Invitrogen | Cat#T3168 |
| Digitonin | EMD Millipore | Cat#11024–24-1 |
| DNeasy Blood and Tissue kit | QIAGEN | Cat#69506 |
| COX8-EGFP-mCherry plasmid | Addgene | Cat#78520 |
| Hs-APOL1 -C probe | bio-techne | Cat#459791 |
| APOL1-No-XMm-C2 | bio-techne | Cat#459798-C2 |
| Mm-Nlrp3 | bio-techne | Cat#439571 |
| Hs-PECAM1-O1 | bio-techne | Cat#487381 |
| Critical Commercial Assays | ||
| Mouse albumin specific ELISA | Bethyl Laboratories | Cat#E99–134 |
| Creatinine reagent | Diazyme | Cat#DZ072B |
| RNAscope 2.5 HD Duplex Detection Kit | bio-techne | Cat#322436 |
| Multi Tissue dissociation kit 1 | Miltenyi | Cat#130–110-2011 |
| APOL1 ELISA Kit | Proteintech | Cat#KE00047 |
| Mouse IL-1 beta ELISA Kit | R&D Systems | MLB00C |
| Mouse TNF-alpha | R&D Systems | MTA00B |
| Il-10 ELISA Kit | R&D Systems | Cat#M1000B |
| Angpt2 ELISA Kit | R&D Systems | Cat#MANG20 |
| Myeloperoxidase assay kit | Sigma | Cat#MAK068–1KT |
| Expermental Models:Organisms/Strains | ||
| Cdh5 tTA | Jackson Laboratory | Stock#013585 |
| GT26.rtTA | Jackson Laboratory | Stock#5670 |
| Nlrp3KO | Jackson Laboratory | Stock#21302 |
| STING KO | Jackson Laboratory | Stock#025805 |
| Caspase1/11 KO | Jackson Laboratory | Stock#16621 |
| Gsdmd KO | Jackson Laboratory | Stock#032410 |
| Alb-Cre | Jackson Laboratory | Stock#003574 |
| TREG0APOL1 | N/A | |
| TREG2APOL1 | N/A | |
| Deposited data | ||
| Raw and analyzed data | This paper | GEO:GSE181671 |
| Seqence-based Reagents | ||
| Primers see Table S2 | This paper | N/A |
| siRNA targeting sequence: Tmem173 (Duplex 1): GAAUCGGGUUUAUUCCAACAGCGTC |
IDT | N/A |
| siRNA targeting sequence: Tmem173 (Duplex 2): UUCUUAGCCCAAAUAAGGUUGUCGCAG |
IDT | N/A |
| Software and Algorithms | ||
| ImageJ | NIH | https://imagej.nih.gov/ij |
| Prism 8 | Graphpad Software | https://www.graphpad.com/scientific-software/prism |