Fig. 1.

Workflow overview. A Strategy for detection and validation of PGC-1α isoforms by SMRT sequencing in mice. Starting from raw data from SMRT sequencing, primary and secondary analyses were performed, resulting in full-length non-concatemers (FLNC). Then, a similarity search against PGC-1α was performed, yielding in 18 potential novel PGC-1α transcripts, resulting in 12 high-fidelity novel PGC-1α isoforms after quality control. B Strategy for investigating into differential expression of PGC-1α isoforms by diet-induced obesity and ischemia/reperfusion (I/R) injury in mice followed by qPCR. Mice fed either a standard chow or high-fat diet underwent I/R or sham surgery. Then, tissue from the infarct area and the remote area (distant from the infarcted area) as baseline as well as 3 and 16 days post I/R was collected and used for expression analysis using qPCR