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. 2022 May 11;34(8):2989–3005. doi: 10.1093/plcell/koac136

Figure 1.

Figure 1

VC-targeted H3K27me3 erasure in the male gametophyte. A, REF6 was expressed under the control of the Lat52 promoter to purposefully erase H3K27me3 in VCs. The transgenic lines were named TL. Whole-mount H3K27me3 antibody labeling and DAPI staining of mature pollen in WT (top) and strong TL-10 line (bottom). Dashed lines indicate VN. B, Fluorescence signal intensity of the WT (left) and TL-10 (right) VN is displayed along the direction of the long white arrow in (A). C, Statistical analysis of the average signal intensity of REF6-GFP and H3K27me3 in the VN of WT and TL-10 pollen. Statistical significance is determined by Student’s t test (***P < 0.001) and the error bars show sd. Scale bars, 2 μm (A).