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. 2022 May 11;34(8):2989–3005. doi: 10.1093/plcell/koac136

Figure 3.

Figure 3

VC-targeted H3K27me3 erasure triggers the VC–SC fate transition. A–C, The MG cell-specific markers in control and TL-10 pollen. Fluorescence images of the MGU stained with DAPI, also indicated by ProLat52:REF6-GFP and ProHTR10:HTR10-RFP (A), ProDUO1:DUO1-RFP (B), and H3K9me2 signals (C), in control and TL-10. Type ① and type ② indicate TL-10 VN without or with MG cell-specific marker signals, respectively. Dotted lines indicate the ectopic HTR10-RFP (A), DUO1-RFP (B) signals, and arrow indicates the ectopic H3K9me2 signals in VN (dashed line) (C). D, Statistical analysis of the ectopic MG cell-specific marker signal in VN. Statistical significance was determined by Student’s t test (***P < 0.001) and the error bars show sd. Scale bars, 2 μm (A–C).