Figure 6.

Inhibition of ROCK1 signal can inactivate ROCK1/NF-κB pathway of MS-treated RAW264.7 macrophages. Protein expression of IL6, iNOS, Rho, ROCK1, ROCK2, NF-κB, Iκb-α, and p-Iκb-α was analyzed by Western blotting after 5%, 10%, and 15% Elong mechanical stretch for 8 h. Control indicates cells were cultured at static condition. The (+) Y27632 group was pretreated with Y27632 (10 μM, 12 h). Control indicates cells were cultured at static condition. Quantitative analysis of protein via the Image J software. The results are expressed as the means ± SD (n = 3). Compare to control in MS, ^∗P < 0.05 and ^∗∗P < 0.01. Compare to control in MS+Y-27632: ^#P < 0.05 and ^##P < 0.01. Compare to MS group, ^∗P < 0.05 and ^∗∗P < 0.01.