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. 2022 Jul 23;2022:6133629. doi: 10.1155/2022/6133629

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Copyright © 2022 Liaoyuan Huang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Berberine activates autophagy in NP cells. (a, b) NP cells were treated with BBR, BBR and IL-1β, or BBR, IL-1β, and 3-MA (10 mM), (a) and protein expressions were analyzed by immunoblot. (b) Relative protein expression was analyzed by ImageJ software. (c) Confocal imaging of NP cells were collected by staining LC3II antibody (green). Scale bars: 25 μm, magnified ×400. The data are present as the mean ± SD of three experiments. ^∗∗P < 0.01 compared with the control group; ^##P < 0.01 compared with the IL-1β group; ^&&P < 0.01 compared with the BBR + IL-1β group.