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. 2022 May 7;39(4):623–639. doi: 10.1007/s10585-022-10169-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — FTO regulates ESCC cell proliferation and migration, invasion and colony formation. A The over-expression efficiency of FTO was identified at RNA and protein levels using PCR (top) and western blotting (bottom). The interference efficiency of FTO was identified at RNA and protein levels by PCR (top) and western blotting (bottom). B CCK-8 assay was used to analyze the proliferation efficiency of cells after interfering with the expression efficiency of FTO. Overexpression of FTO (FTO-OE) significantly increased the proliferation efficiency of HEEC cells, while inhibition of FTO (sh-FTO) significantly decreased the proliferation efficiency of KYSE150 cells. C Transwell assay was used to assay the invasion and migration ability of HEEC cells. Overexpression of FTO (FTO-OE) significantly increased the invasion and migration ability of HEEC cells, while inhibition of FTO (sh-FTO) significantly decreased the invasion and migration ability of KYSE150 cells. D Colony formation assay was used to analyze the clone formation ability of KYSE150 cells after inhibition or overexpression of FTO. Results were presented as means ± SD (n = 3 per group). The two-tailed Student’s t-test, one-way ANOVA and two-way ANOVA were used to perform comparison between two groups and more groups, respectively. *p < 0.05, ***p < 0.001