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. 2022 May 7;39(4):623–639. doi: 10.1007/s10585-022-10169-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — ERBB2 regulates the invasion and metastasis of ESCC cells and reverses biological effects of FTO. A, B RT-PCR and western blot was used to analyze the mRNA and protein level of ERBB2 in KYSE150 cells. C The CCK-8 assays showed that down-regulation of ERBB2 could inhibit the proliferation of KYSE150 cells. D, E Transwell assay was used to analyze the ability of down-regulation of ERBB2 to inhibit KYSE150 cell invasion and migration. The bar chart shows the corresponding quantitative analysis (right). F Western blot experiment verified the efficiency of the up-regulation ERBB2 expression and the promotion of EMT by the up-regulation ERBB2 expression. G The up-regulation of ERBB2 expression restored the down-regulation of FTO to the inhibition of ERBB2 expression using RT-PCR. H CCK-8 assay confirmed that up-regulation ERBB2 expression restored down-regulated FTO to KYSE150 cell proliferation inhibition. I, K and H Migration (I) invasion (K) and colony formation (H) assays showed that up-regulation of ERBB2 expression restored down-regulated FTO to KYSE150 cell migration, invasion and proliferation inhibition. The bar chart shows the corresponding quantitative analysis (right). The data was presented as the mean ± SD (n = 3). The two-tailed Student’s t-test, one-way ANOVA and two-way ANOVA were used to perform comparison between two groups and more groups, respectively. n.s no statistical significance. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001