Fig. 7.

FTO and ERBB2 regulates in vivo ESCC progression. A The effects of FTO and ERBB2 on tumor formation, tumor weight and tumor volume change in nude mouse KYSE150-derived xenograft model. Representative images of tumors from FTO knockdown (sh-FTO) and up regulation expression ERBB2 (ERBB2-OE) versus the negative control sh-NC and NC-OE, respectively (n = 3 for each group).FTO knockdown effectively decreased ESCC subcutaneous tumor formation and growth. Overexpression of ERBB2 effectively increased ESCC subcutaneous tumor formation and growth. B The expression of Vimentin and E-cadherin, were analyzed from sh-NC, sh-FTO, NC-OE and ERBB2-OE KYSE150-derived xenograft by IHC. C Wide type (WT), FTO knockdown (sh-FTO), stable up regulation expression ERBB2 (ERBB2-OE) and FTO knockdown simultaneously stable up regulation expression ERBB2 (sh-FTO+ERBB2-OE) from KYSE150 cells were injected into the nude mice by tail vein injection. Representative images of metastatic lung tumors and the H&E staining results were shown (left), and the number of lung tumors was quantitatively analyzed (right). Results were presented as means ± SD (n = 4 per group). The two-tailed Student’s t-test and one-way ANOVA were used to compare the difference between two groups and more groups, respectively. n.s no statistical significance. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001