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. 2022 Jul 30;13(7):663. doi: 10.1038/s41419-022-05120-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A–D A representative western blot and relevant quantification of Bax, Bcl-2 and Cleaved Caspase-3 in mouse podocytes (n = 5). E The relative expression of Bcl-2/Bax ratio in mouse podocytes. F, G The mRNA levels of Bax and Bcl-2 were analyzed by RT-qPCR in mouse podocytes (n = 5). H, I TUNEL staining results for podocytes under different conditions (n = 5). J, K The apoptosis rate of podocytes was detected by flow cytometry (n = 3). NG: 5.6 mM d-glucose; M: 5.6 mM d-glucose+24.4 mM mannitol; HG: 30 mM d-glucose; HG + C: HG + control shRNA plasmid; HG + OE: HG + Sestrin2 pcDNA; HG + shRNA: HG + Sestrin2 shRNA plasmid. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. TUNEL terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.