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. 2022 Jul 30;13(7):663. doi: 10.1038/s41419-022-05120-0

Fig. 4. Sestrin2 ameliorates HG-induced oxidative stress and activation of TSP-1/TGF-β1/Smad3 signaling pathway in podocytes.

Fig. 4

A–C The protein and mRNA level of Nox4 was detected by western blot and RT-qPCR in podocytes (n = 5). D–H The protein levels of TSP-1, TGF-β1, CD36, and phosphorylation level of Smad3 were examined by western blot in podocytes (n = 5). I–L RT-qPCR was used to detect the mRNA levels of TSP-1, TGF-β1, CD36, and Smad3 in podocytes (n = 5). M ROS production of podocytes cultured under different conditions was determined by flow cytometry. NG: 5.6 mM d-glucose; M: 5.6 mM d-glucose+24.4 mM mannitol; HG: 30 mM d-glucose; HG + C: HG + control shRNA plasmid; HG + OE: HG + Sestrin2 pcDNA; HG + shRNA: HG + Sestrin2 shRNA plasmid. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.