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. 2022 Jul 30;12:13135. doi: 10.1038/s41598-022-17446-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Ym155 decreases mitochondrial respiration: The Seahorse XFe24 analyzer was used to monitor oxygen consumption rate (OCR) and examine oxidative phosphorylation of H1299 cells treated with Ym155 for 3 h. Inhibitors of ETC oligomycin (complex V), FCCP (uncoupler), Rotenone (complex I) were used to interrogate oxidative phosphorylation. (A) OCR curves of H1299 cells treated with Ym155. (B) Mean basal respiration, (C) ATP production, (D) maximal respiration, (E) proton leak, and (F) spare capacity from 3 experiments.