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. 2022 Jul 30;13:4435. doi: 10.1038/s41467-022-32176-5

Fig. 1. RNA-binding protein RBM3 is highly expressed by lung ILCs during fungal allergen exposure and is regulated by IL-33 and TSLP.

Fig. 1

a Wild-type mice were challenged 4 times with 50 µg Alternaria over the span of 10 days and four ILC subsets (CD127+ST2+, CD127−ST2+, CD127+ST2−, CD127−ST2−) were sorted and collected for RNA-seq. Heatmap showing the relative expression of the top 25 most highly expressed RBPs in the four ILC subtypes. RBPs involved in the cell cycle or splicing were excluded. Statistics were performed with Centroid linkage and Manhattan clustering. b Absolute transcript levels of the top 10 RBPs in the four ILC subtypes. Statistics were performed with an unpaired t-test, two-tailed. Data representative of 2 samples (p < 0.01). c RBM3 mRNA levels of ILC2s in comparison to several traditional ILC2 mRNA (IL-5, IL-13, Gata-3, Rora, and CysLT1R) and non-ILC2 mRNAs (Rorgt and Tbet). ILC2s were from Alternaria challenged mice. Data are from triplicate samples and analyzed with an unpaired t-test, two-tailed. d RBM3 expression on ILC2s from naive, PBS challenged, or Alternaria challenged lung. Black = Naive, Blue = PBS challenged, Red = Alternaria challenged (24 h). e RBM3 expression on lung ILC2s of Tslpr−/− mice and mice treated with anti-IL-33R antibody. Data representative of at least 4 mice (paired) per group. Mice were challenged with Alternaria four times. Grey = isotype control, Black = Wild-Type or IgG treated, Red = Tslpr−/− or anti-IL-33R treated. *p < 0.05, **p < 0.01, ***p < 0.001. Data are presented as mean values+/− SEM.