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. 2022 Jul 30;12:13131. doi: 10.1038/s41598-022-16274-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of DNA damage in LN-18 using alkaline comet assay. (A) DNA damage expressed as tail moment in cells treated respectively with IC₅₀ values of FLDP-5, FLDP-8 and curcumin at different time-points ranging from 30 min until 6-h. Fluorescence microscopic images stained with EtBr stain of untreated cells (a), cells treated with FLDP-5 at 2.5 μM for 4-h (b), FLDP-8 at 5 μM for 4-h (c), curcumin at 25 μM for 4-h (d), FLDP-5 at 2.5 μM for 6-h (e), FLDP-8 at 5 μM for 6-h (f), curcumin at 25 μM for 6-h (g) and positive control (h). Each data was obtained from three independent experimental replicates and each data point in (A) was expressed as mean ± SEM of tail moment. *p < 0.05 against negative control, NEG and # p < 0.05 against curcumin.