Figure 7.

Assessment of cell invasion in LN-18 cells using Boyden chamber transwell assay. (A) LN-18 cells were treated respectively with IC₅₀ and IC₂₅ of FLDP-5, FLDP-8 curcuminoid analogues and curcumin at 24-h and the number of invaded of cells was measured at 560 nm. (B) Microscopic images of untreated cells with serum, untreated cells without serum, cells treated with FLDP-5 curcuminoid analogue (1.25 μM and 2.5 μM), FLDP-8 curcuminoid analogue (2.5 μM and 5 μM) and curcumin (12.5 μM and 25 μM) for 24-h. Each data was obtained from three independent experimental replicates and each data point was expressed as mean ± SEM of percentage of relative invasion. *p < 0.05 against negative control, NEG.