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. 1999 Jan;181(2):389–395. doi: 10.1128/jb.181.2.389-395.1999

TABLE 3.

Properties of predicted gene products in the R. meliloti phn gene cluster

Gene Shine-Dalgarno sequencea Predicted size of product
Closest homolog of predicted product Properties
Amino acids Molecular mass (kDa)
phnG GGAG (4) 155 16.6 E. coli PhnG protein; 37% identity and 77% similarity over 148-aab overlap
phnH GGAGAGGA (3) 199 21.2 E. coli PhnH protein; 37% identity and 73% similarity in 188-aa overlap
phnI GGAG (7) 367 40.8 E. coli PhnI protein; 57% identity and 88% similarity in 354-aa overlap Contains a sequence with similarity to lipoxygenase pattern 2 sequence
phnJ AGGAG (7) 297 33.4 E. coli PhnJ protein; 68% identity and 97% similarity in 275-aa overlap Has four conserved cysteines, a potential metal-binding site
phnK AGGAGG (7) 258 28.6 E. coli PhnK protein; 68% identity and 90% similarity in 253-aa overlap HisP-like nucleotide-binding protein
a

Numbers in parentheses indicate nucleotide spacing from the putative Shine-Dalgarno sequence to the initiating methionine. 

b

aa, amino acid.