TABLE 3.
Properties of predicted gene products in the R. meliloti phn gene cluster
Gene | Shine-Dalgarno sequencea | Predicted size of product
|
Closest homolog of predicted product | Properties | |
---|---|---|---|---|---|
Amino acids | Molecular mass (kDa) | ||||
phnG | GGAG (4) | 155 | 16.6 | E. coli PhnG protein; 37% identity and 77% similarity over 148-aab overlap | |
phnH | GGAGAGGA (3) | 199 | 21.2 | E. coli PhnH protein; 37% identity and 73% similarity in 188-aa overlap | |
phnI | GGAG (7) | 367 | 40.8 | E. coli PhnI protein; 57% identity and 88% similarity in 354-aa overlap | Contains a sequence with similarity to lipoxygenase pattern 2 sequence |
phnJ | AGGAG (7) | 297 | 33.4 | E. coli PhnJ protein; 68% identity and 97% similarity in 275-aa overlap | Has four conserved cysteines, a potential metal-binding site |
phnK | AGGAGG (7) | 258 | 28.6 | E. coli PhnK protein; 68% identity and 90% similarity in 253-aa overlap | HisP-like nucleotide-binding protein |
Numbers in parentheses indicate nucleotide spacing from the putative Shine-Dalgarno sequence to the initiating methionine.
aa, amino acid.