TABLE 4.
Abilities of Rm1021 and Pn1 R. meliloti strains to grow on a various phosphorus sources compared with results of previous studies with E. coli
Phosphorus source | Growth ofa:
|
||
---|---|---|---|
Rm1021 | Pn1 | E. coli (reference[s]) | |
Aminoethylphosphonate | +++b | +++b | G (23, 33) |
Aminomethylphosphonate | +++b | −b | |
3-Aminopropylphosphonate | ++ | − | |
Glyphosate | ++b | −b | NG (18) |
Methylphosphonate | ++b | −b | G (10) |
Ethylphosphonate | ++ | − | G (10) |
Propylphosphonate | ++ | − | G (42) |
tert-butylphosphonate | − | − | NG (10) |
Phenylphosphonate | ++ | − | G (10) |
Benzylphosphonate | ++ | − | NG (10) |
4-Aminobenzylphosphonate | ++ | − | |
Phosphonoacetate | +b | −b | G (33) |
Glycerol-3-phosphate | +++ | +++ |
+++, similar rate of growth and final OD as the positive control culture growing with 0.5 mM KH2PO4 as a phosphorus source in liquid minimal medium; ++, lower rate of growth than the positive control culture but reaching the same final OD; +, lower rate of growth than the positive control culture and lower final OD; −, similar rate of growth and final OD as the negative control containing no added phosphorus source; G, growth; NG, no growth.
Result confirmed on minimal medium solidified with phosphate-free agarose.